#3 Preparing your does for Artificial Insemination When considering Artificial Insemination, you will want to use a synchronization program on your does so you can do and monitor the progress efficiently. Doing this, you will want to make sure your does are all in good condition, updated in vaccinations, and in good health to ensure a successful operation. The first couple of weeks after AI are the most important to keep your does happy. Even a small fever or stress can cause them to abort, cost