May 19, 2023 · Here, we develop genetically immortalized bovine satellite cells (iBSCs) via constitutive expression of bovine Telomerase reverse transcriptase (TERT) and Cyclin-dependent kinase 4 (CDK4). These cells achieve over 120 doublings at the time of publication and maintain their capacity for myogenic differentiation.

Dec 3, 2022 · In the present work, primary bovine satellite cells (BSCs) are engineered via transposon integration of TERT and CDK4 to establish immortalized bovine myogenic cells (iBSCs).

Dec 11, 2024 · For immortal porcine MSCs, human telomerase reverse transcriptase (hTERT) gene was overexpressed in parental cells to restore telomerase activity and lengthen cell longevity. After selection, G418-resistant cells were expanded and …

Sep 14, 2011 · Herein we described a new conditionally immortal satellite cell derived cell-line, H2K 2B4, which was generated from the H-2Kb -tsA58 immortomouse. The H2K 2B4 clone was extensively characterised in vitro and in vivo and was shown to exhibit muscle stem cell characteristics in both environments.

Dec 22, 2022 · We show that lecithin activates peroxisome proliferator-activated receptor gamma (PPARγ), inducing adipogenesis in immortalized fibroblasts. Blending cultured adipocyte-like cells with extruded soy...

A new conditionally immortal satellite cell-derived cell-line, H2K 2B4, was generated from the H2K(b)-tsA58 immortomouse. Under permissive conditions H2K 2B4 cells terminally differentiate in vitro to form uniform myotubes with a myogenic …

Non-treated H2K SF1 cells were transplanted into the contralateral irradiated TA muscle. As a control for myoblast engraftment efficiency, a wild type conditionally immortal satellite cell derived cell-line, H2K 2B4 , was injected into both irradiated TA muscles of an mdx nu/nu mouse.

Bypassing cellular senescence and becoming immortal is a prerequisite step in the tumorigenic transformation of a cell. It has long been known that loss of a key tumor suppressor gene, such as p53, is necessary, but not sufficient, for spontaneous cellular immortalization.

Dec 3, 2022 · Here, we develop genetically immortalized bovine satellite cells (iBSCs) via constitutive expression of bovine Telomerase reverse transcriptase (TERT) and Cyclin-dependent kinase 4 (CDK4). These cells achieve over 120 doublings at the time of publication and maintain their capacity for myogenic differentiation.

Jun 20, 2024 · A peculiar cluster of stars swirling around the violent center of the Milky Way could become "immortal" by continuously capturing and destroying dark matter particles in their cores, a new study...