Sequence-independent amplification methods allow for the detection of novel viruses that are too divergent to be detected by other methods. These methods use random priming or the ligation of ...

PCR, or Polymerase Chain Reaction, is a revolutionary molecular biology technique that allows for the rapid amplification of specific DNA sequences. It is an essential tool in various fields, ...

RAPD is a multilocus approach that uses low-stringency PCR conditions to permit promiscuous amplification of genomic regions. It can be carried out in the absence of genome sequence data ...

Note what amount of tailed cDNA generated the largest quantity of amplified 5′ termini. The identity of the band can be confirmed by DNA sequencing, Southern hybridization and/or restriction ...