Protein A/protein G–Sepharose beads (50:50 mix; Sigma) were added and incubation was continued for 1–2 h at 4 °C. Precipitates were washed four times with IPH buffer (1 ml), separated on 10% ...

opbg.net Background: Metastatic spread in retinoblastoma is a rare occurrence in developed countries but still associated with a poor prognosis. Patients and methods: Medical records of all metastatic ...

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