To amplify the target DNA, they used strand displacement amplification (SDA), an isothermal reaction that results in total displacement of the target strand after each amplification reaction.

We call this expanding cascade of strand displacement and fragment-generation events 'DNA hyperbranching', and the special rolling circle amplification driven by two primers 'Hyperbranched-RCA ...

and loop-mediated isothermal amplification (LAMP). WGA, particularly employing the multiple strand displacement amplification (MDA) technique, allows the generation of large quantities of genomic DNA ...