A 1.4-Mb tandem duplication, including the gene for peripheral myelin protein 22 (PMP22) in chromosome 17p11.2-12 is responsible for 70% of the cases of the demyelinating type 1 of Charcot-Marie-Tooth ...

PCR-based techniques have transformed how scientists analyze transcripts’ translational applications in cancer and ...

You have full access to this article via your institution. So the question remains "What are the essential prerequisites for establishing a quantitative RT-PCR assay?" To date, a great deal of effort ...

Polymerase Chain Reaction (PCR) is a method which utilizes the mechanism of DNA replication to amplify a specific part of DNA. This involves the use of DNA polymerase, which makes new strands of DNA ...

Quantitative polymerase chain reaction, or "qPCR", is considered the “second generation” of PCR and was first described in 1992. The past three decades have seen significant advances in qPCR ...

SalivaDirect, an open-source protocol, avoids many of the supply bottlenecks of other tools and could be offered for as little as $10 a test. From flies landing on food to the retraction of the ...

Quantitative PCR (qPCR) and digital PCR (dPCR) are powerful tools for nucleic acid detection and quantification, each with distinct strengths. Both technologies play vital roles in genomics research, ...

What is real-time quantitative PCR? qPCR uses one of two methods to detect the changes to DNA amplification: use of fluorescent dyes or sequence-specific DNA probes. qPCR is normally performed in a ...

In the six decades since Watson and Crick identified the double helical structure of DNA, a series of elegant and powerful experiments has established the broad outlines of how genetic information is ...