How it Works: Real Time PCR Brendan Maher The instrumentation is basic: a thermal cycler for amplification, a light source for excitation of fluorescent probes (see chemistries below), a camera for ...

Since its invention in the 1980s, polymerase chain reaction (PCR) opened the door for applications such as genetic engineering, genotyping, and sequencing, and has become a fundamental part of ...

Once the concept of PCR popped into Kary Mullis’ head in 1983 it wasn’t long before scientists realized they had a revolutionary technology in their hands. Because now they could take the smallest ...

Rad, explores how droplet digital PCR (ddPCR) overcomes limitations of traditional PCR, reshaping how scientists approach ...