Nanoscopy is a field of microscopy that focuses on imaging and studying structures and processes at the nanoscale, typically below the diffraction limit of light. It encompasses various techniques ...

What is Single-Molecule Localization Microscopy? Single-molecule localization microscopy (SMLM) is a super-resolution imaging technique that enables the visualization of biological structures and ...

The photo-kinetics of fluorescent molecules have enabled the circumvention of the far-field optical diffraction limit. Despite its enormous potential, the necessity to label the sample may adversely ...

Researchers from the University of Science and Technology of China (USTC) have unveiled a planar optical device that significantly enhances the capabilities of dark-field microscopy, achieving ...

Attempts to break the diffraction limit with 'super lenses' have all hit the hurdle of extreme visual losses. Now physicists have shown a new pathway to achieve superlensing with minimal losses, ...

Researchers have developed a new measurement and imaging approach that can resolve nanostructures smaller than the diffraction limit of light. After light interacts with a sample, the new technique ...

Typically in optical microscopy, the signal-to-noise ratio (SNR) is calculated to quantify the image contrast which indicates how well different regions of interest in the image can be differentiated.

It’s relatively easy to understand how optical microscopes work at low magnifications: one lens magnifies an image, the next magnifies the already-magnified image, and so on until it reaches the eye ...

ISM is a super-resolution imaging technique that enhances resolution beyond the diffraction limit through pixel reassignment and deconvolution. However, the practical implementation of ISM requires ...

Standard optical microscopes have surrendered their once dominant position at the forefront of scientific research to more advanced tools. As we delve deeper into the microscopic world, photons just ...

In this article, we look at some of the key developments of various microscopy techniques over the last few years and how they have benefitted different areas of research and development. The impact ...

Researchers from the University of Science and Technology of China (USTC) led by Prof. ZHANG Douguo have unveiled a planar optical device that significantly enhances the capabilities of dark-field ...