The epigenetic modification, DNA methylation, is characterized by 5-methylcytosine (5-mC) and found in specific genomic ...
1| Add 0.001–100 ng of poly(A) + mRNA or 0.01–1 μg of total RNA into each of three microcentrifuge tubes. Adjust the volumes to 9 μl with water. Denature the RNA by heating at 75 °C for 5 ...
Rolling-circle amplification (RCA) driven by DNA polymerase can replicate circularized oligonucleotide probes with either linear or geometric kinetics under isothermal conditions. In the presence ...